Surreptitiously projecting different movies to two subsets of viewers.

A new technique (Manipulation of Overlapping Rivalrous Images by polarizing filters, MORI) was invented for presenting two different images on the same screen that can be seen separately by two groups of viewers without them noticing the overlap. It can easily create desired artificial conflicts among viewers. Two polarizing filters perpendicular to each other provide separate invisible channels from dual video projectors to two groups of viewers via a single screen. The basic principle of the presentation technique, details of the apparatus, and limitations were introduced. As an example of the application of this technique, an eyewitness experiment was briefly reported. The results of experiments conducted by the author and colleagues provide evidence of the effectiveness of this technique with various projectors, video materials, group sizes, and ages of participants

Imaging Ca2+ Concentration and pH in Nanopores/Channels of Protein Crystals

Protein crystals are nanoporous materials. Despite this important characteristic, little is known about the conditions in the pores, also called channels. Here, we describe a method to study the calcium concentration and pH in the nanopores of thaumatin and lysozyme crystals. We load the crystal nanopores with fluorescent indicators and then perfuse the crystals with solutions of different calcium concentrations and pH while reading out the crystal’s fluorescence intensity with confocal microscopy. By calibrating the fluorescence signal, we can determine the calcium concentration and pH in the nanopores. For the pH in thaumatin nanopores measured with the ratiometric pH sensor SNARF-1, we find a −0.7 pH shift compared to the bath pH corresponding to a fivefold higher proton concentration. This is similar to the −0.3 pH shift found in lysozyme nanopores. With single-wavelength probes, we find that the calcium concentration in thaumatin crystal nanopores is the same as in the bath, whereas it is 0.24 times lower in lysozyme nanopores. Summarizing, our experiments show that calcium concentration and pH in the nanopores of protein crystals can deviate significantly from that in the bath. In general, the described method can be applied for testing a wide range of ion or small-molecule concentrations in transparent nanoporous materials not only with ratiometric but also with single wavelength fluorescent indicators

Protective effects of fermented rice vinegar sediment (Kurozu moromimatsu) in a diethylnitrosamine-induced hepatocellular carcinoma animal model

Kurozu moromimatsu is the sediment of Kurozu, a jar-fermented Japanese black vinegar produced from unpolished rice. Here, we examined the protective effects of Kurozu moromimatsu in a diethylnitrosamine-induced model of hepatocellular carcinoma. Thirty-two F344 rats were divided into two groups; the control group received basal CE-2 diet, and the Kurozu moromimatsu group received CE-2 diet containing Kurozu moromimatsu. At 16 weeks after initial intraperitoneal administration of diethylnitrosamine (150 mg/kg/week), serum was collected from half the rats. These rats were sacrificed and the liver was resected for histological examination of hematoxylin-eosin-stained sections and assay of matrix metalloproteinase-2 and matrix metalloproteinase-9 levels in tumor tissues. Glutathione S-transferase placental form-positive foci were evaluated by immunostaining for glutathione S-transferase placental form. The remaining rats were maintained for evaluation of survival. There were no significant differences of serum transaminases, tumor necrosis factor-alpha, and also no marked hepatic histological differences, between the two groups. However, the size of hepatocellular carcinomas was greatly decreased and the levels of activated matrix metalloproteinase-2 and -9 were significantly reduced in the Kurozu moromimatsu group. Further, survival was significantly prolonged in the Kurozu moromimatsu group compared with the control. These results indicate that Kurozu moromimatsu inhibited the growth of hepatocellular carcinoma

A Case of Cystic Basal Cell Carcinoma Which Shows a Homogenous Blue/Black Area under Dermatoscopy

Basal cell carcinoma (BCC) is the most common skin tumor and contains several different histopathological types. Here, we report a case of cystic basal cell carcinoma, which is relatively rare and might be clinically misdiagnosed. A dermatoscopic examination of the case revealed a homogenous blue/black area usually not seen in BCC. We reviewed 102 BCC cases resected and diagnosed at Sapporo Medical University Hospital between April 2005 and March 2010. Among them, only three were the cystic type

Performance of a newly developed SDCCD for X-ray use

A Scintillator Deposited CCD (SDCCD) is a wide-band X-ray detector consisting of a CCD and a scintillator directly attached to each other. We assembled the newly developed SDCCD that the scintillator CsI(Tl) is below the fully depleted CCD. The incident X-rays enter the CCD depletion layer first. Then, X-rays passing through the depletion layer are absorbed in the CsI(Tl). The contact surface of the CCD is a back-illuminated side so that we can have good light collection efficiency. In our experimental setup, we confirmed good performance of our SDCCD detecting many emission lines up to 88\,keV that comes from $^{109}$Cd.Comment: 4 pages, 6 figures, accepted publication for Nucl. Instr. and Meth. (2010